Dhaniel Abdi Wicaksana
Brawijaya University School of Medicine
Title: The role of ß-glucan in the diagnosis of fungal chronic rhinosinusitis
Biography
Biography: Dhaniel Abdi Wicaksana
Abstract
Background: Fungal chronic rhinosinusitis is a major health problem because of the large impacts associated with declining quality of life and difficult medical treatment as well as the prevalence that tends to increase. There is currently opportunity to establish diagnosis without invasive intervention by utilizing β-glucan which is the largest component of fungal cell wall. Purpose: To discover the congruity of β-glucan level in paranasal sinus tissue and blood serum as a potentially diagnosis marker for fungal chronic rhinosinusitis patients. Methods: This was a cross sectional study which involved 20 subjects. Blood sampling and maxillary sinus surgery were performed, then fungi identification in the sinus mucosa was done by polymerase chain reaction PCR technique. If a fungi species with β-glucan was found, then the examination was continued with the measurement of β-glucan by enzyme-linked immunosorbent assay (ELISA) technique in sinus and blood. Results: Aspergillus flavus is the most commonly found fungus. All subjects passed the positive β-glucan limit (≥ 80 pg/mL) of the mucosal sample and only 1 subject had intermediate results (60-79 pg/mL) from blood. Paired t test result was no significant difference between the level of β-glucan in blood and mucosal sinus (p=0.886), so that β-glucan examination of blood can describe β-glucan levels in paranasal sinuses. Conclusion: β-glucan may be used to establish the diagnosis of fungal chronic rhinosinusitis so it is hoped that the process of diagnosis of chronic rhinosinusitis fungus can be obtained quickly and precisely without the need for invasive procedure although it requires more research, especially related to diagnostic test.